Chemiluminescence immunoassay of aldosterone in serum.

Stabilization of glucose in blood specimens: mechanism of delay in fluoride inhibition of glycolysis.

1. Wion-Barbot N, Schuffenecker I, Niccoli P, Conte-Devolx B, Lecomte P, Houdent C, et al. Results of the calcitonin stimulation test in normal volunteers compared with genetically unaffected members of MEN 2A and familial medullary thyroid carcinoma families. Ann Endocrinol (Paris) 1997;58:302–8. 2. Motte P, Vauzelle P, Gardet P, Ghillani P, Caillou B, Parmentier C, et al. Construction and cli...

Enzyme Immunoassay for the Quantitative Determination of TESTOSTERONE in Human Serum or plasma INTRODUCTION OF CHEMILUMINESCENCE IMMUNOASSAY

Effect of ATP-Dependent K+ Channel Openers and Blockers on Serum Concentration of Aldosterone in Rats

There are many reports for involvement of ATP-sensitive potassium channels in pancreatic, cardiac and vascular smooth muscle cells. This study examined the effect of single doses of K+ channel openers diazoxide, minoxidil and K+ channel blockers chlorpropamide, glibenclamide on serum concentration of aldosterone in male rats. Blood samples were obtained 60 minutes after drug treatment and serum...

Solid-phase chemiluminescence immunoassay for progesterone in unextracted serum.

We describe a simple, solid-phase chemiluminescence immunoassay for progesterone in 10 microL of unextracted serum ("direct" assay). Danazol at pH 8.0 is included (100 ng per tube) to displace progesterone from binding proteins in serum. A progesterone-11 alpha-hemisuccinyl-aminobutylethyl isoluminol conjugate serves as the chemiluminescent ligand marker and homologous antiprogesterone IgG cova...

Protein-enhanced fluorescein chemiluminescence used in an immunoassay for rubella antibody in serum.

The chemiluminescence of fluorescein derivatives is greatly enhanced when they are conjugated to proteins. We exploited this property in devising a chemiluminescence immunoassay for rubella antibodies. In this sequential "sandwich"-type assay, tubes coated with rubella antigen are used for antibody separation and goat antibody to human IgG conjugated with fluorescein isothiocyanate is used for ...